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For these reasons, species-specific and cell line-specific actions of TRPA1 channel activators such as JWU-A021 must be taken into account when planning high throughput screening approaches targeted at the identification of new GLP-1 secretagogues
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In addition, neutral endopeptidase (NEP) plays a minor role in GLP-1 degradation by cleaving at six sites, Asp15-Val16, Ser18-Tyr19, Tyr19-Leu20, Glu27-Phe28, Phe28-Ile29 and Trp31-Leu32, within the central and C-terminal peptide domains [44]
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